Addgene - pMIG-hSOX2 Plasmid Data

Plasmid 17226: pMIG-hSOX2

Gene/insert name:
SRY (sex determining region Y)-box 2
Alt name:
SOX2
Insert size:
954
Species:
H. sapiens (human)
Entrez Gene:
SOX2 (ANOP3, MCOPS3)
Vector backbone:
pMIG
(Search Vector Database)
Vector type:
Retroviral
Backbone size w/o insert (bp):
6500
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
George Daley
Terms and Licenses:
MTA

Comments:

Addgene has confirmed the presence of GFP in the pMIG vector backbone by sequencing, but the functionality of the GFP has not been confirmed.

Please visit www.addgene.org/18119 to find the paper in which the pMIG-cMyc was used

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Reprogramming of human somatic cells to pluripotency with defined factors. Park et al (Nature. 2007 Dec 23. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17226" in your Materials and Methods section.