- Backbone size w/o insert (bp) 6500
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Gene/Insert nameSRY (sex determining region Y)-box 2
SpeciesH. sapiens (human)
Insert Size (bp)954
Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
Addgene has confirmed the presence of GFP in the pMIG vector backbone by sequencing, but the functionality of the GFP has not been confirmed.
Please visit www.addgene.org/18119 to find the paper in which the pMIG-cMyc was used
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIG-hSOX2 was a gift from George Daley (Addgene plasmid # 17226)
For your References section:Reprogramming of human somatic cells to pluripotency with defined factors. Park IH, Zhao R, West JA, Yabuuchi A, Huo H, Ince TA, Lerou PH, Lensch MW, Daley GQ. Nature. 2007 Dec 23. ():. 10.1038/nature06534 PubMed 18157115
Map generated by Addgene from full sequence.