- Backbone size w/o insert (bp) 6500
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Gene/Insert nameKruppel-like factor 4 (gut)
SpeciesH. sapiens (human)
Insert Size (bp)1413
Entrez GeneKLF4 (a.k.a. EZF, GKLF)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
The hKlf4 in this plasmid matches GenBank's protein AAH30811.1, and also has an S240G amino acid change.
Addgene has confirmed the presence of GFP in the pMIG vector backbone by sequencing, but the functionality of the GFP has not been confirmed.
Note that the pMIG-cMyc used in this paper can be found here: www.addgene.org/18119 .
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIG-hKLF4 was a gift from George Daley (Addgene plasmid # 17227)
For your References section:Reprogramming of human somatic cells to pluripotency with defined factors. Park IH, Zhao R, West JA, Yabuuchi A, Huo H, Ince TA, Lerou PH, Lensch MW, Daley GQ. Nature. 2007 Dec 23. ():. 10.1038/nature06534 PubMed 18157115
Map generated by Addgene from full sequence.