1
Plasmid 17227: pMIG-hKLF4
  • Kruppel-like factor 4 (gut)

  • KLF4

  • 1413

  • H. sapiens (human)

  • KLF4 (RP11-150J11.1, EZF, GKLF)

  • pMIG
    (Search Vector Database)

  • Mammalian Expression, Retroviral

  • 6500

  • EcoRI

  • No

  • XhoI

  • No

  • pLXSN 5' List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • View sequences (1)
  • George Daley

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

The hKlf4 in this plasmid matches GenBank's protein AAH30811.1, and also has an S240G amino acid change.

Addgene has confirmed the presence of GFP in the pMIG vector backbone by sequencing, but the functionality of the GFP has not been confirmed.

Note that the pMIG-cMyc used in this paper can be found here: www.addgene.org/18119 .

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Reprogramming of human somatic cells to pluripotency with defined factors. Park et al (Nature. 2007 Dec 23. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17227" in your Materials and Methods section.