pMIG-hKLF4
(Plasmid #17227)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pMIG
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Kruppel-like factor 4 (gut)
  • Alt name
    KLF4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1413
  • Entrez Gene
    KLF4 (a.k.a. RP11-150J11.1, EZF, GKLF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The hKlf4 in this plasmid matches GenBank's protein AAH30811.1, and also has an S240G amino acid change.

Addgene has confirmed the presence of GFP in the pMIG vector backbone by sequencing, but the functionality of the GFP has not been confirmed.

Note that the pMIG-cMyc used in this paper can be found here: www.addgene.org/18119 .

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIG-hKLF4 was a gift from George Daley (Addgene plasmid # 17227)
  • For your References section:

    Reprogramming of human somatic cells to pluripotency with defined factors. Park IH, Zhao R, West JA, Yabuuchi A, Huo H, Ince TA, Lerou PH, Lensch MW, Daley GQ. Nature. 2007 Dec 23. ():. 10.1038/nature06534 PubMed 18157115