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MSCV h c-MYC IRES GFP
(Plasmid #18119)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18119 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV IRES GFP
  • Backbone manufacturer
    Dr. Robert Hawley of George Washington University
  • Backbone size w/o insert (bp) 5660
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    c-MYC
  • Alt name
    pMIG-cMYC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1858
  • Mutation
    The CUG which is the start site for Myc I is present, however, there is a 3bp deletion between this and the ATG start site for Myc II.
  • GenBank ID
    NM_002467
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The MSCV plasmid is owned and was provided by Dr. Robert Hawley of George Washington University. The IRES-GFP DNA was developed by Dr. Arthur Nienhuis of St. Jude Children's Research Hospital (SJCRH). Dr. John L. Cleveland cloned the human c-myc gene into the construct while at SJCRH. Dr. Cleveland obtained an MTA for this construct from SJCRH when he moved to the Scripps Research Institute, Florida.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

The MSCV vector is described in; Hawley, R.G., Lieu, F.H.L., Fong, A.Z.C., and Hawley, T.S. Versatile retroviral vectors for potential use in gene therapy. Gene Ther. 1: 136-138, 1994.
Use of the construct is described in; Park, I., Zhao, R., West, J.A., Yabuuchi, A., Huo, H., Ince, T.A., Lerou, P.H., Lensch, M.W., and Daley, G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 451, 141-146, 2008.

Please note that the assembled sequence presented here is not 100% reflective of the actual sequence. Addgene has found another HindIII site at the end of the MYC gene (see our quality control sequence).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV h c-MYC IRES GFP was a gift from John Cleveland (Addgene plasmid # 18119)