Addgene - MSCV h c-MYC IRES GFP Plasmid Data

Plasmid 18119: MSCV h c-MYC IRES GFP

Gene/insert name:
c-MYC
Alt name:
pMIG-cMYC
Insert size:
1858
Species:
H. sapiens (human)
GenBank ID:
NM_002467
Entrez Gene:
MYC (MRTL, bHLHe39, c-Myc)
Fusion protein or tag:
GFP
Terminal:
C terminal on backbone
Mutation:
The CUG which is the start site for Myc I is present, however, there is a 3bp deletion between this and the ATG start site for Myc II.
Vector backbone:
MSCV IRES GFP
(Search Vector Database)
Backbone manufacturer:
Dr. Robert Hawley of George Washington University
Vector type:
Retroviral
Backbone size w/o insert (bp):
5660
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
The MSCV plasmid is owned and was provided by Dr. Robert Hawley of George Washington University. The IRES-GFP DNA was developed by Dr. Arthur Nienhuis of St. Jude Children's Research Hospital (SJCRH). Dr. John L. Cleveland cloned the human c-myc gene into the construct while at SJCRH. Dr. Cleveland obtained an MTA for this construct from SJCRH when he moved to the Scripps Research Institute, Florida.
Sequence: View sequences (2)
Map:
View map
Supplemental document:
MSCV-h-cMyc-IRES-GFP seq (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
John Cleveland
Terms and Licenses:
MTA

Comments:

The MSCV vector is described in; Hawley, R.G., Lieu, F.H.L., Fong, A.Z.C., and Hawley, T.S. Versatile retroviral vectors for potential use in gene therapy. Gene Ther. 1: 136-138, 1994.
Use of the construct is described in; Park, I., Zhao, R., West, J.A., Yabuuchi, A., Huo, H., Ince, T.A., Lerou, P.H., Lensch, M.W., and Daley, G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 451, 141-146, 2008.

Please note that the assembled sequence presented here is not 100% reflective of the actual sequence. Addgene has found another HindIII site at the end of the MYC gene (see our quality control sequence).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18119" in your Materials and Methods section.