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Noninvasive optical inhibition with a red-shifted microbial rhodopsin.
Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sorensen AT, Young A, Klapoetke NC, Henninger MA, Kodandaramaiah SB, Ogawa M, Ramanlal SB, Bandler RC, Allen BD, Forest CR, Chow BY, Han X, Lin Y, Tye KM, Roska B, Cardin JA, Boyden ES
Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014 Jul 6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
65012fck-Jaws-KGC-GFP-ER2Lentivirus-mediated expression of Jaws under the CaMKII promoter.
65013fck-Jaws-GFP-ER2Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.
65014pAAV-hsyn-Jaws-KGC-GFP-ER2AAV-mediated expression of Jaws under the human synapsin promoter
65015pAAV-CaMKII-Jaws-KGC-GFP-ER2AAV-mediated expression of Jaws under the CaMKII promoter
65016pAAV-hsyn-Jaws-GFP-ER2AAV-mediated expression of Jaws-ER2 under the human synapsin promoter
78174pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]AAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.
84445pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
84446pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
99233pAAV-CAG-Jaws-KGC-GFP-ER2AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter. Using bGH pA signal.
108274pAAV-EF1α1.1-FLEX-rc [Jaws-KGC-GFP-ER2]AAV-mediated expression of Jaws-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
11154920XUAS IVS Jaws mCherry_trOptogenetics in Drosophila: UAS-induced expression of Jaws mCherry
11155010XUAS IVS Jaws mCherry_trOptogenetics in Drosophila: UAS-induced expression of Jaws mCherry
11155113XLexAop2 IVS Jaws mCherry_trOptogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherry
11155220XUAS IVS Jaws mVenus_trOptogenetics in Drosophila: UAS-induced expression of Jaws mVenus
11155310XUAS IVS Jaws mVenus_trOptogenetics in Drosophila: UAS-induced expression of Jaws mVenus
153534AAV-CaMKII-Jaws-KGC-tdTomato-ER2AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII promoter.
153535AAV-Syn-Jaws-KGC-tdTomato-ER2AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter.
153536pAAV-Syn-FLEX-rc [Jaws-KGC-tdTomato-ER2]AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner.
153537pAAV-EF1α1.1-Jaws-KGC-GFP-ER2AAV-mediated expression of Jaws-GFP-ER2 under the EF1α promoter (1.1kb short version).
153538pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version).
195488AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.
196140AAV-CAG-CreAAV-mediated expression of Cre under the CAG promoter .

Antibodies from Article