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High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells.
Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W
Nature. 2014 Apr 9. doi: 10.1038/nature13166.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
53118pLenti-OC-IRES-BSDTo create stable cell clones with high-level expression of Cas9 and OCT1
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53121pLenti-sgRNA-LibsgRNA expression construction in a 3rd generation lentiviral backbone
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Antibodies from Article