Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.
Port F, Chen HM, Lee T, Bullock SL
Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 49408 | pCFD1-dU6:1gRNA | expression of gRNA under control of the Drosophila U6:1 promoter |
| 49409 | pCFD2-dU6:2gRNA | expression of gRNA under control of the Drosophila U6:2 promoter |
| 49410 | pCFD3-dU6:3gRNA | expression of gRNA under control of the Drosophila U6:3 promoter |
| 49411 | pCFD4-U6:1_U6:3tandemgRNAs | expression of two gRNAs from Drosophila U6:1 and U6:3 |
| 62208 | pnos-Cas9-nos | Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster. |
| 62209 | pAct-Cas9 | Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster. |