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pnos-Cas9-nos
(Plasmid #62208)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62208 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pnos
  • Backbone manufacturer
    Johannes Bischof and Konrad Basler
  • Backbone size w/o insert (bp) 9200
  • Total vector size (bp) 13353
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cas9
  • Promoter nanos

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATAAAGAAGTATCGCGAATAC
  • 3′ sequencing primer TTACTATCTATCTGGTTAACCC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    hCas9 from George Church (Addgene plasmid 41815). pnos backbone from Johannes Bischof and Konrad Basler.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

Please visit crisprflydesign.org for more information.

Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pnos-Cas9-nos was a gift from Simon Bullock (Addgene plasmid # 62208 ; http://n2t.net/addgene:62208 ; RRID:Addgene_62208)
  • For your References section:

    Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Port F, Chen HM, Lee T, Bullock SL. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. 10.1073/pnas.1405500111 PubMed 25002478