The Susan Lindquist laboratory of the Howard Hughes Medical Institute and the Whitehead Institute for Biomedical Research has deposited a set of Gateway destination vectors for high-throughput genetic analysis in S. cerevisiae. Academic and non-profit laboratories may request these vectors from Addgene for research and educational use.

These plasmids have been described in

A suite of Gateway cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae. (Yeast. 2007 Jun 21;)
Simon Alberti, Aaron D. Gitler, and Susan Lindquist. (PubMed)

Description

This kit contains 288 yeast Gateway destination vectors. The vectors are based on the widely used pRS series of yeast shuttle vectors and contain the two commonly used GPD and GAL1 promoter expression systems that enable expression of ORFs either constitutively or under galactose-inducible conditions. Additionally, proteins of interest can be fused to a choice of frequently used N or C terminal tags, such as EGFP, ECFP, EYFP, Cerulean, DsRed, HA, or TAP.

Search: Tag: Any ECFP EGFP EYFP 3HA TAP Cerulean Marker: Any HIS3 TRP1 LEU2 URA3

Browse Plasmids

Download Plasmid Sequences in Tab Delimited Format

 
Please note that the full sequences for these plasmids are predicted. Addgene has sequenced the promoter, tag, and selectable marker region of each plasmid for verification. In a few cases, the sequencing results do not perfectly match the predicted sequences. Sequencing results are available from each plasmid's datasheet.

Distribution

The kit is $800, plus shipping and handling. This kit will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80 degrees Celsius immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid (with discounts for bulk orders), and will be shipped as bacterial stabs.

96-well Plates: Plate 1, Plate 2, Plate 3.

Download Information and Plate Map PDF (included with shipment)

 
Note that Addgene uses DHL for shipments and can ship dry ice to most countries, excluding China. Scientists from China interested in obtaining this kit should contact help@addgene.org.

"Gateway" is a registered trademark of Invitrogen.