|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14153||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||14153-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Vector typeYeast Expression ; Gateway Destination
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsDB3.1, ccdB Survival
- Cloning method Gateway Cloning
- 5′ sequencing primer GAL1 (Common Sequencing Primers)
Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Information for Cloning Grade DNA (Catalog # 14153-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAG425GAL-ccdB was a gift from Susan Lindquist (Addgene plasmid # 14153 ; http://n2t.net/addgene:14153 ; RRID:Addgene_14153)