pTP223
(Plasmid #13263)

• Depositing Lab: Kenan Murphy
• Publication: Murphy et al BMC Mol Biol. 2003 Dec 13. 4():11.

Backbone

• Vector backbone: pMC7
• Backbone size w/o insert (bp): 6000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Plac-gam-bet-exo
• Alt name: Lambda Red
• Species: bacteriophage lambda
• Insert Size (bp): 2000

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Anthony Poteete
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original description can be found in Poteete & Fenton (1984) Virology, 134:161-167.