Addgene - pKM208 Plasmid Data

Plasmid 13077: pKM208

Gene/insert name:
lacI repressor
Insert size:
1400
Species:
E. coli
GenBank ID:
J01636
Entrez Gene:
lacI (b0345, ECK0342, JW0336)
Vector backbone:
pKM201
(Search Vector Database)
Backbone manufacturer:
Kenan Murphy
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
7200
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
30
Growth instructions:
Plasmid contains temperature sensitive origin of replication and must be grown at 30C
High or low copy:
Low Copy
Person or lab that originally
cloned the gene/insert:
Anthony Poteete UMass Medical School
Sequence: View sequences (3)
Principal Investigator:
Kenan Murphy
Terms and Licenses:
MTA

Comments:

lacI gene obtained from plasmid pTP550

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli. Murphy et al (BMC Mol Biol. 2003 Dec 13. 4():11. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13077" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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