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pKM208
(Plasmid #13077)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13077 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pKM201
  • Backbone manufacturer
    Kenan Murphy
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid contains temperature sensitive origin of replication and must be grown at 30C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    lacI repressor
  • Species
    E. coli
  • Insert Size (bp)
    1400
  • GenBank ID
    J01636
  • Entrez Gene
    lacI (a.k.a. b0345, ECK0342)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Anthony Poteete UMass Medical School
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

lacI gene obtained from plasmid pTP550

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM208 was a gift from Kenan Murphy (Addgene plasmid # 13077 ; http://n2t.net/addgene:13077 ; RRID:Addgene_13077)
  • For your References section:

    Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli. Murphy KC, Campellone KG. BMC Mol Biol. 2003 Dec 13. 4():11. 10.1186/1471-2199-4-11 PubMed 14672541