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pKD4
(Plasmid #45605)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45605 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pANTSγ
  • Backbone manufacturer
    M. Koob (University of Wisconsin, Madison)
  • Total vector size (bp) 3267
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5a lambda pir
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FRT-kan-FRT
  • Promoter R6K

Cloning Information

Resource Information

Depositor Comments

This is a template plasmids for frt-flanked kan cassette. The kan cassette originally came from pCP15.

pKD3 and pKD4 are identical except for the region between the FRT sites, so they create an identical scar that has stop codons in all six reading frames. As drawn in the map, this scar has an idealized ribosome binding site and start codon for downstream (rightward) gene expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKD4 was a gift from Barry L. Wanner (Addgene plasmid # 45605 ; http://n2t.net/addgene:45605 ; RRID:Addgene_45605)
  • For your References section:

    One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Datsenko KA, Wanner BL. Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. 10.1073/pnas.120163297 PubMed 10829079