Ngn3 Cre (DM#259)
(Plasmid #15023)

• Depositing Lab: Douglas Melton
• Publication: Gu et al Development. 2002 May . 129(10):2447-57.

Backbone

• Vector backbone: pBluescript II KS
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression, Cre/Lox ; mouse transgenic

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ngn3 promoter
• Alt name: pGD84
• Species: M. musculus (mouse)
• Insert Size (bp): 7900
• Entrez Gene: Neurog3 (a.k.a. Atoh5, Math4B, bHLHa7, ngn3)
• Tag / Fusion Protein:
  • Cre (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pMC-Cre was a gift from Andrew McMahon.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library. The Ngn3-Cre construct was obtained by directly ligating a XbaI-BssHII (partial digestion) blunt-ended fragment from pGD15 to the pMC-Cre plasmid to generate pGD 84. The insert was released by NotI-SalI digestion and used for transgenic animal production.

See Author's Map and article for more information.