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pBS185 CMV-Cre
(Plasmid #11916)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    cre
  • Species
    bacteriophage P1
  • Insert Size (bp)
    1100
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

pBS185 carries the wt cre gene under the control of the major immediate early promoter and enhancer of human cytomegalovirus (hCMV). This is a strong promoter that gives fairly generalized expression. However, it is not a strong promoter in embryonic stem (ES) cells. The construct also carries the mouse metallothionein MT-I region including the polyadenylation site and several introns. For higher level expression in general and for better expression in ES cells plasmid pBS513 is superior. The sequence shown includes cre and the metallothionein region.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS185 CMV-Cre was a gift from Brian Sauer (Addgene plasmid # 11916)
  • For your References section:

    Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. Sauer B, Henderson N. New Biol. 1990 May . 2(5):441-9. PubMed 2288914