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Plasmid 11916: pBS185 CMV-Cre
  • Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter

  • cre

  • 1100

  • bacteriophage P1

  • cre (P1_gp003)

  • na
    (Search Vector Database)

  • Mammalian Expression, Cre/Lox

  • 6000

  • na List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (3)
  • View map

  • Brian Sauer

  • UBMTA

Comments: 

pBS185 carries the wt cre gene under the control of the major immediate early promoter and enhancer of human cytomegalovirus (hCMV). This is a strong promoter that gives fairly generalized expression. However, it is not a strong promoter in embryonic stem (ES) cells. The construct also carries the mouse metallothionein MT-I region including the polyadenylation site and several introns. For higher level expression in general and for better expression in ES cells plasmid pBS513 is superior. The sequence shown includes cre and the metallothionein region.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. Sauer et al (New Biol. 1990 May . 2(5):441-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11916" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only