Addgene - pBS598 EF1alpha-EGFPcre Plasmid Data

Plasmid 11923: pBS598 EF1alpha-EGFPcre

Gene/insert name:
EGFP-cre
Alt name:
cre
Insert size:
1800
Species:
bacteriophage P1
Entrez Gene:
cre (P1_gp003)
Vector backbone:
na
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert (bp):
6600
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (2)
Principal Investigator:
Brian Sauer
Terms and Licenses:
MTA

Comments:

pBS598 carries the EGFPcre fusion gene under the control of the strong elongation factor EF1alpha promoter. This promoter expresses at high levels in most cell types, including embryonic stem (ES) cells. However its use in transgenic mice is not recommended: the promoter is often silenced in a transgene setting. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression.

Note: pBS513 (EF1alpha-cre) is very similar to pBS598 but carries the wt cre gene and thus serves as the non-fluorescent counterpart of pBS598.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: GFPcre fusion vectors with enhanced expression. Le et al (Anal Biochem. 1999 Jun 1. 270(2):334-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11923" in your Materials and Methods section.