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pBS513 EF1alpha-cre
(Plasmid #11918)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11918 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cre
  • Species
    bacteriophage P1
  • Insert Size (bp)
    1100
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

Resource Information

Depositor Comments

pBS513 carries the wt cre gene under the control of the elongation factor-1 alpha (EF-1 alpha) promoter. The construct carries an upstream Kozak modification to increase expression. The resulting construct provides very strong expression in many different types of cells, including murine embryonic stem (ES) cells. For cell culture work this construct is recommended as a replacement for the CMV-cre construct pBS185 as it provides much stronger expression. However, pBS513 is not suitable for use in transgenic mice as such EF-1 alpha transgene constructs are often inactive in many tissues.

Please note: The following enzymes are NOT single cutters: PstI, AflIII, and SacI. We do not suggest using these enzymes for cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS513 EF1alpha-cre was a gift from Brian Sauer (Addgene plasmid # 11918 ; http://n2t.net/addgene:11918 ; RRID:Addgene_11918)
  • For your References section:

    GFPcre fusion vectors with enhanced expression. Le Y, Miller JL, Sauer B. Anal Biochem. 1999 Jun 1. 270(2):334-6. 10.1006/abio.1999.4110 PubMed 10334853