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RIP Cre-ER (DM#265)
(Plasmid #15029)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15029 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    modified pBluescript II KS
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Mammalian Expression, Cre/Lox ; for making transgenic constructs

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rat insulin promoter (RIP)
  • Alt name
    insulin promoter
  • Alt name
    ins2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    700
  • Entrez Gene
    Ins2 (a.k.a. CP-II)
  • Tag / Fusion Protein
    • Cre-ER (TM) (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cre-ER(TM) was a gift from Andy McMahon.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.

The RIP–CreER construct was generated by fusing a 0.66-kilobase SmaI–HindIII fragment of the RIP2 promoter to a minimal hsp68 promoter, and placing the chimaeric promoter upstream of CreER(TM).

See Author's Map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RIP Cre-ER (DM#265) was a gift from Douglas Melton (Addgene plasmid # 15029 ; http://n2t.net/addgene:15029 ; RRID:Addgene_15029)
  • For your References section:

    Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Dor Y, Brown J, Martinez OI, Melton DA. Nature. 2004 May 6. 429(6987):41-6. 10.1038/nature02520 PubMed 15129273