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pGL410_INS421
(Plasmid #49057)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49057 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL4.10[luc2]
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4242
  • Total vector size (bp) 4614
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human INS promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    410
  • Promoter INS promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer RVprimer4
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL410_INS421 was a gift from Kevin Ferreri (Addgene plasmid # 49057 ; http://n2t.net/addgene:49057 ; RRID:Addgene_49057)
  • For your References section:

    Insulin gene expression is regulated by DNA methylation. Kuroda A, Rauch TA, Todorov I, Ku HT, Al-Abdullah IH, Kandeel F, Mullen Y, Pfeifer GP, Ferreri K. PLoS One. 2009 Sep 9;4(9):e6953. doi: 10.1371/journal.pone.0006953. 10.1371/journal.pone.0006953 PubMed 19742322