pGL410_INS421
(Plasmid #49057)

• Purpose: Expresses luciferase under control of human insulin gene promoter
• Depositing Lab: Kevin Ferreri
• Publication: Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10.1371/journal.pone.0006953.

Backbone

• Vector backbone: pGL4.10[luc2]
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4242
• Total vector size (bp): 4614
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human INS promoter
• Species: H. sapiens (human)
• Insert Size (bp): 410
• Entrez Gene: INS (a.k.a. IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4)
• Promoter: INS promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: RVprimer4

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry