Addgene - Cerulean Plasmid Data

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Plasmid 15214: Cerulean

Gene/insert name:		monomeric Cerulean
Alternative names:		mCER
Insert size (bp):		731
Species of gene(s):		synthetic
Relevant mutations/deletions:		A206K
Vector backbone:		pEGFP-C1 (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Mammalian expression
Backbone size (bp):		4000
Cloning site 5':		NheI
Site destroyed during cloning:		No
Cloning site 3':		Bsr GI
Site destroyed during cloning:		No
5' Sequencing primer:		EGFP-N (List of Sequencing Primers)
3' Sequencing primer:		EGFP-C
Bacteria resistance:		Kanamycin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Dave Piston
Terms and Licenses:		MTA

Comments: The EGFP in pEGFP-C1 was replaced with Cerulean.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: An improved cyan fluorescent protein variant useful for FRET. Rizzo MA et al. (Nat Biotechnol. 2004 Apr . 22(4):445-9. Pubmed)

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