Addgene - cytoplasmic EKAR (Cerulean-Venus) Plasmid Data

Plasmid 18679: cytoplasmic EKAR (Cerulean-Venus)

Gene/insert name:
ERK Activity Reporter
Alt name:
EKAR
Insert size:
550
Fusion protein or tag:
monomeric Cerulean
Terminal:
N terminal on insert
Fusion protein or tag:
monomeric Venus
Terminal:
C terminal on insert
Vector backbone:
pRK5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4500
5' sequencing primer:
CMV Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
David Yue (Glycine linker) David Litchfield (Pin1) Deborah Morrison (Cdc25C)
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Karel Svoboda
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A genetically encoded fluorescent sensor of ERK activity Harvey et al (PNAS 2008 Dec 9;105(49):19264-9 PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18679" in your Materials and Methods section.