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nuclear EKAR (EGFP-mRFP)
(Plasmid #18682)


Item Catalog # Description Quantity Price (USD)
Plasmid 18682 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    ERK Activity Reporter
  • Alt name
  • Insert Size (bp)
  • Tags / Fusion Proteins
    • EGFP (N terminal on insert)
    • mRFP (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nuclear EKAR (EGFP-mRFP) was a gift from Karel Svoboda (Addgene plasmid # 18682 ; ; RRID:Addgene_18682)
  • For your References section:

    A genetically encoded fluorescent sensor of ERK activity. Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, Svoboda K.. PNAS 2008 Dec 9;105(49):19264-9 10.1073/pnas.0804598105 PubMed 19033456