nuclear EKAR (EGFP-mRFP)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18682||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameERK Activity Reporter
Insert Size (bp)550
/ Fusion Proteins
- EGFP (N terminal on insert)
- mRFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDavid Yue (Glycine linker) David Litchfield (Pin1) Deborah Morrison (Cdc25C)
Terms and Licenses
Article Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:nuclear EKAR (EGFP-mRFP) was a gift from Karel Svoboda (Addgene plasmid # 18682)
For your References section:A genetically encoded fluorescent sensor of ERK activity. Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, Svoboda K.. PNAS 2008 Dec 9;105(49):19264-9 10.1073/pnas.0804598105 PubMed 19033456
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.