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pBI-MCS-EGFP
(Plasmid #16542)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16542 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The tet-responsive reporter plasmid pBI-EGFP was purchased from Clontech and modified by the inclusion of a polylinker to create pBI-MCS-EGFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBI-MCS-EGFP was a gift from Bert Vogelstein (Addgene plasmid # 16542 ; http://n2t.net/addgene:16542 ; RRID:Addgene_16542)
  • For your References section:

    Identification and classification of p53-regulated genes. Yu J, Zhang L, Hwang PM, Rago C, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1999 Dec 7. 96(25):14517-22. 10.1073/pnas.96.25.14517 PubMed 10588737