Addgene - pLenti CMV rtTA3 Blast (w756-1) Plasmid Data

Plasmid 26429: pLenti CMV rtTA3 Blast (w756-1)

Gene/insert name:
Tetracycline repressor A3 mutant
Alt name:
rtTA3
Insert size:
707
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
7681
5' sequencing primer:
CMVforw List of Sequencing Primers
3' sequencing primer:
WPRErev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC.
High or low copy:
High Copy
Selectable markers:
Blasticidin
Person or lab that originally
cloned the gene/insert:
The rtTA3 element was a gift from Dr. Dominic Esposito.
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

Use to make them Tet-On Advanced.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence difference does not affect expression of the rtTA3.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26429" in your Materials and Methods section.