pLenti CMV rtTA3 Blast (w756-1)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 7681
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsStbl3, 37oC.
Copy numberHigh Copy
Gene/Insert nameTetracycline repressor A3 mutant
Insert Size (bp)707
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVforw
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe rtTA3 element was a gift from Dr. Dominic Esposito.
Terms and Licenses
Use to make them Tet-On Advanced.
There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence difference does not affect expression of the rtTA3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMV rtTA3 Blast (w756-1) was a gift from Eric Campeau (Addgene plasmid # 26429)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.