pLenti CMV rtTA3 Blast (w756-1)
(Plasmid #26429)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 7681
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3, 37oC.
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Tetracycline repressor A3 mutant
  • Alt name
    rtTA3
  • Insert Size (bp)
    707

Cloning Information

Resource Information

Depositor Comments

Use to make them Tet-On Advanced.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence difference does not affect expression of the rtTA3.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMV rtTA3 Blast (w756-1) was a gift from Eric Campeau (Addgene plasmid # 26429)