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Plasmid 26429: pLenti CMV rtTA3 Blast (w756-1)
  • 3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast 

  • Tetracycline repressor A3 mutant

  • rtTA3

  • 707

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 7681

  • CMVforw List of Sequencing Primers

  • WPRErev

  • Ampicillin

  • Stbl3

  • 37

  • Stbl3, 37oC.

  • High Copy

  • Blasticidin

  • The rtTA3 element was a gift from Dr. Dominic Esposito.

  • View sequences (3)
  • View map

  • Eric Campeau

  • UBMTA
    Tet Systems Limited Use Label License
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Use to make them Tet-On Advanced.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence difference does not affect expression of the rtTA3.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26429" in your Materials and Methods section.