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Plasmid 26429: pLenti CMV rtTA3 Blast (w756-1)
  • 3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast 

  • Tetracycline repressor A3 mutant

  • rtTA3

  • 707

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 7681

  • CMVforw List of Sequencing Primers

  • WPRErev

  • Ampicillin

  • Stbl3

  • 37

  • Stbl3, 37oC.

  • High Copy

  • Blasticidin

  • The rtTA3 element was a gift from Dr. Dominic Esposito.

  • View sequences (3)
  • View map

  • Eric Campeau

  • UBMTA
    Tet Systems Limited Use Label License
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Use to make them Tet-On Advanced.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence difference does not affect expression of the rtTA3.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26429" in your Materials and Methods section.