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pLenti CMV rtTA3 Blast (w756-1)
(Plasmid #26429)

  • Purpose
    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast 
  • Depositing Lab
  • Price
    $65 (USD)
    Shipped as bacteria in an agar stab at ambient temperature
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Available to Academic and Nonprofits Only


  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 7681
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Stbl3, 37oC.
  • Copy number
    High Copy

Sequence Information


  • Gene/Insert name
    Tetracycline repressor A3 mutant
  • Alt name
  • Insert Size (bp)

Cloning Information

Resource Information

Depositor Comments

Use to make them Tet-On Advanced.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence difference does not affect expression of the rtTA3.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMV rtTA3 Blast (w756-1) was a gift from Eric Campeau (Addgene plasmid # 26429)