pLenti CMVtight Hygro DEST (w769-1)
(Plasmid #26433)

Available to Academic and Nonprofits Only


  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size (bp) 10104
  • Vector type
    Mammalian Expression, Lentiviral ; Tet-On Advanced Destination vector
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Chloramphenicol
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    DB3.1 or ccDB survival, 37oC
  • Copy number
    Low Copy

Sequence Information


  • Gene/Insert name
    no insert

Cloning Information

Resource Information

Depositor Comments

Need to be transduced in a Tet-On advanced cell line in order to obtain expression of the GOI.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.

Addgene found this plasmid to be extremely slow growing.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMVtight Hygro DEST (w769-1) was a gift from Eric Campeau (Addgene plasmid # 26433)