pLenti CMVtight Hygro DEST (w769-1)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 10104
Vector typeMammalian Expression, Lentiviral ; Tet-On Advanced Destination vector
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Chloramphenicol
Growth Strain(s)ccdB Survival
Growth instructionsDB3.1 or ccDB survival, 37oC
Copy numberLow Copy
Gene/Insert nameno insert
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVtightFORW
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe CMVtight element was a gift from Dr. Dominic Esposito.
Terms and Licenses
Need to be transduced in a Tet-On advanced cell line in order to obtain expression of the GOI.
There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.
Addgene found this plasmid to be extremely slow growing.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMVtight Hygro DEST (w769-1) was a gift from Eric Campeau (Addgene plasmid # 26433)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.