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Plasmid 26433: pLenti CMVtight Hygro DEST (w769-1)
  • no insert

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral ; Tet-On Advanced Destination vector

  • 10104

  • CMVtightFORW List of Sequencing Primers

  • WPRErev

  • Ampicillin and Chloramphenicol

  • ccdB Survival

  • 37

  • DB3.1 or ccDB survival, 37oC

  • Low Copy

  • Hygromycin

  • The CMVtight element was a gift from Dr. Dominic Esposito.

  • View sequences (2)
  • View map

  • Notes from Addgene (1)

  • Eric Campeau

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Need to be transduced in a Tet-On advanced cell line in order to obtain expression of the GOI.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.

Addgene found this plasmid to be extremely slow growing.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26433" in your Materials and Methods section.