1
Plasmid 26433: pLenti CMVtight Hygro DEST (w769-1)
  • no insert

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral ; Tet-On Advanced Destination vector

  • 10104

  • CMVtightFORW List of Sequencing Primers

  • WPRErev

  • Ampicillin and Chloramphenicol

  • ccdB Survival

  • 37

  • DB3.1 or ccDB survival, 37oC

  • Low Copy

  • Hygromycin

  • The CMVtight element was a gift from Dr. Dominic Esposito.

  • View sequences (2)
  • View map

  • Notes from Addgene (1)

  • Eric Campeau

  • MTA

Comments: 

Need to be transduced in a Tet-On advanced cell line in order to obtain expression of the GOI.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.

Addgene found this plasmid to be extremely slow growing.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26433" in your Materials and Methods section.