pLenti CMVtight Puro DEST (w768-1)
(Plasmid #26430)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size (bp) 9402
  • Vector type
    Mammalian Expression, Lentiviral ; Tet-On Advanced Destination vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    DB3.1 or ccDB survival, 37oC.
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    no insert

Cloning Information

Resource Information

Depositor Comments

Need to be transduced in a Tet-On advanced cell line to obtain expression of the GOI.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMVtight Puro DEST (w768-1) was a gift from Eric Campeau (Addgene plasmid # 26430)