1
Plasmid 26430: pLenti CMVtight Puro DEST (w768-1)
  • no insert

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral ; Tet-On Advanced Destination vector

  • 9402

  • CMVtightFORW List of Sequencing Primers

  • WPRErev

  • Ampicillin and Chloramphenicol

  • ccdB Survival

  • 37

  • DB3.1 or ccDB survival, 37oC.

  • Low Copy

  • Puromycin

  • The CMVtight element was a gift from Dr. Dominic Esposito.

  • View sequences (3)
  • View map

  • Eric Campeau

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Need to be transduced in a Tet-On advanced cell line to obtain expression of the GOI.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26430" in your Materials and Methods section.