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Plasmid 26430: pLenti CMVtight Puro DEST (w768-1)
  • no insert

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral ; Tet-On Advanced Destination vector

  • 9402

  • CMVtightFORW List of Sequencing Primers

  • WPRErev

  • Ampicillin and Chloramphenicol

  • ccdB Survival

  • 37

  • DB3.1 or ccDB survival, 37oC.

  • Low Copy

  • Puromycin

  • The CMVtight element was a gift from Dr. Dominic Esposito.

  • View sequences (3)
  • View map

  • Eric Campeau

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Need to be transduced in a Tet-On advanced cell line to obtain expression of the GOI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26430" in your Materials and Methods section.