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pLenti CMV rtTA3 Hygro (w785-1)
(Plasmid #26730)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26730 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 8669
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3, 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tetracycline repressor A3 mutant
  • Alt name
    rtTA3
  • Insert Size (bp)
    707

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site Pvu II (destroyed during cloning)
  • 5′ sequencing primer CMVforw
  • 3′ sequencing primer WPRErev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid can be used to generate Tet-On advanced cell lines. It has been reported that it works
better than other tetracycline repressor (Markusic et al. 2005).

This plasmid has a mismatch in the first attB site. The consequences of this mismatch are not clear, however depositor successfully made 2 different cell lines with that construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMV rtTA3 Hygro (w785-1) was a gift from Eric Campeau (Addgene plasmid # 26730 ; http://n2t.net/addgene:26730 ; RRID:Addgene_26730)