pDB2
(Plasmid #18954)

• Depositing Lab: Michele Calos
• Publication: Keravala et al J Gene Med. 2006 Aug . 8(8):1008-17.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4735
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phiC31 attB
• Alt name: int
• Alt name: phiC31 bacterial attachment site
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 312
• GenBank ID: X60952

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (destroyed during cloning)
• 3′ cloning site: MluI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry