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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 18935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMVSPORTbetaGal
- Backbone size w/o insert (bp) 4357
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namephiC31 integrase
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SpeciesStreptomyces phage phiC31
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Insert Size (bp)1873
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Entrez Geneint (a.k.a. phiC31p51)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bythe plasmid pIJ8600 (a gift from Mervyn Bibb, John Innes Institute, Norwich, U.K.)
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMVInt was a gift from Michele Calos (Addgene plasmid # 18935 ; http://n2t.net/addgene:18935 ; RRID:Addgene_18935) -
For your References section:
A phage integrase directs efficient site-specific integration in human cells. Groth AC, Olivares EC, Thyagarajan B, Calos MP. Proc Natl Acad Sci U S A. 2000 May 23. 97(11):5995-6000. 10.1073/pnas.090527097 PubMed 10801973