Addgene - pInt Plasmid Data

Plasmid 18941: pInt

Gene/insert name:
phiC31 integrase
Insert size:
2182
Species:
Streptomyces phage phiC31
Entrez Gene:
int ()
Mutation:
under lacZ promoter
Vector backbone:
pACYC177
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3016
Cloning site 5':
PstI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Person or lab that originally
cloned the gene/insert:
phiC31 integrase from the plasmid pIJ8600 (Mervyn Bibb, John Innes Institute, Norwich, U.K.)
Sequence: View sequences (2)
Principal Investigator:
Michele Calos
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A phage integrase directs efficient site-specific integration in human cells. Groth et al (Proc Natl Acad Sci U S A. 2000 May 23. 97(11):5995-6000. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18941" in your Materials and Methods section.