Addgene - pBCPB+ Plasmid Data

Plasmid 18940: pBCPB+

Gene/insert name:
attP, lacZ, attB
Insert size:
4031
Vector backbone:
pBCSK+
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3190
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
SpeI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Chloramphenicol
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
attP sequence from the plasmid pIJ8600 (a gift from Mervyn Bibb, John Innes Institute, Norwich, U.K.)
Sequence: View sequences (2)
Principal Investigator:
Michele Calos
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A phage integrase directs efficient site-specific integration in human cells. Groth et al (Proc Natl Acad Sci U S A. 2000 May 23. 97(11):5995-6000. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18940" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

18935: pCMVInt

18941: pInt

18942: pET11phiC31poly(A)

18939: pTA-attP

13795: pPGKPhiC31obpA