Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pET11phiC31poly(A)
(Plasmid #18942)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18942 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET11
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5632
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phiC31 integrase
  • Species
    Streptomyces phage phiC31
  • Insert Size (bp)
    1879
  • Entrez Gene
    int (a.k.a. phiC31p51)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    the plasmid pIJ8600 from Mervyn Bibb, John Innes Institute, Norwich, U.K.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11phiC31poly(A) was a gift from Michele Calos (Addgene plasmid # 18942 ; http://n2t.net/addgene:18942 ; RRID:Addgene_18942)
  • For your References section:

    Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth AC, Fish M, Nusse R, Calos MP. Genetics. 2004 Apr . 166(4):1775-82. 10.1534/genetics.166.4.1775 PubMed 15126397