Addgene - pET11phiC31poly(A) Plasmid Data

Plasmid 18942: pET11phiC31poly(A)

Gene/insert name:
phiC31 integrase
Insert size:
1879
Species:
Streptomyces phage phiC31
Entrez Gene:
int ()
Vector backbone:
pET11
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5632
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
the plasmid pIJ8600 from Mervyn Bibb, John Innes Institute, Norwich, U.K.
Sequence: View sequences (2)
Principal Investigator:
Michele Calos
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth et al (Genetics. 2004 Apr . 166(4):1775-82. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18942" in your Materials and Methods section.