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pBS130
(Plasmid #26290)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26290 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC HSP-Delta23
  • Backbone size w/o insert (bp) 6427
  • Vector type
    Bacterial Expression, Insect Expression ; Drosophila transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phiC31 integrase
  • Species
    Streptomyces phage phiC31
  • Insert Size (bp)
    1839
  • Entrez Gene
    int (a.k.a. phiC31p51)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer gcttcgtctacggagcgaca
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

There is one nucleotide deletion in the promoter region of Addgene sequence that does not alter function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS130 was a gift from Tom Clandinin (Addgene plasmid # 26290 ; http://n2t.net/addgene:26290 ; RRID:Addgene_26290)
  • For your References section:

    A versatile in vivo system for directed dissection of gene expression patterns. Gohl D, Silies M, Gao X, Bhalerao S, Luongo F, Lin CC, Potter C, Clandinin T. Nature Methods (2011) doi:10.1038/nmeth.1561 10.1038/nmeth.1561