Addgene - pBS130 Plasmid Data

Plasmid 26290: pBS130

Gene/insert name:
phiC31 integrase
Insert size:
1839
Species:
Streptomyces phage phiC31
Entrez Gene:
int ()
Vector backbone:
pUC HSP-Delta23
(Search Vector Database)
Vector type:
Bacterial Expression, Insect Expression ; Drosophila transgenesis
Backbone size w/o insert (bp):
6427
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
gcttcgtctacggagcgaca List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Tom Clandinin
Terms and Licenses:
MTA

Comments:

There is one nucleotide deletion in the promoter region of Addgene sequence that does not alter function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile in vivo system for directed dissection of gene expression patterns Gohl et al (Nature Methods (2011) doi:10.1038/nmeth.1561)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26290" in your Materials and Methods section.