|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26290||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepUC HSP-Delta23
- Backbone size w/o insert (bp) 6427
Vector typeBacterial Expression, Insect Expression ; Drosophila transgenesis
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namephiC31 integrase
SpeciesStreptomyces phage phiC31
Insert Size (bp)1839
Entrez Geneint (a.k.a. phiC31p51)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer gcttcgtctacggagcgaca (Common Sequencing Primers)
There is one nucleotide deletion in the promoter region of Addgene sequence that does not alter function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS130 was a gift from Tom Clandinin (Addgene plasmid # 26290 ; http://n2t.net/addgene:26290 ; RRID:Addgene_26290)
For your References section:A versatile in vivo system for directed dissection of gene expression patterns. Gohl D, Silies M, Gao X, Bhalerao S, Luongo F, Lin CC, Potter C, Clandinin T. Nature Methods (2011) doi:10.1038/nmeth.1561 10.1038/nmeth.1561