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pDB2
(Plasmid #18954)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18954 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4735
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phiC31 attB
  • Alt name
    int
  • Alt name
    phiC31 bacterial attachment site
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    312
  • GenBank ID
    X60952

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (destroyed during cloning)
  • 3′ cloning site MluI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDB2 was a gift from Michele Calos (Addgene plasmid # 18954 ; http://n2t.net/addgene:18954 ; RRID:Addgene_18954)
  • For your References section:

    PhiC31 integrase mediates integration in cultured synovial cells and enhances gene expression in rabbit joints. Keravala A, Portlock JL, Nash JA, Vitrant DG, Robbins PD, Calos MP. J Gene Med. 2006 Aug . 8(8):1008-17. 10.1002/jgm.928 PubMed 16779871