pHR'-NRSF-CITE-GFP
(Plasmid #21310)

• Depositing Lab: Jay Nadeau
• Publication: Nadeau et al J Neurophysiol. 2002 Jul . 88(1):409-21.

Backbone

• Vector backbone: pHR'
• Backbone manufacturer: Didier Trono
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: neuron restrictive silencer factor
• Alt name: NRSF, REST
• Species: M. musculus (mouse)
• Insert Size (bp): 3664
• Mutation: None
• GenBank ID: NM_011263
• Entrez Gene: Rest (a.k.a. 2610008J04Rik, NRSF, REST4)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (bicistronic with CITE/IRES)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gift of David Anderson, Caltech reference SHIMOJO M, PAQUETTE AJ, ANDERSON DJ, AND HERSH LB. Protein kinase A regulates cholinergic gene expression in PC12 cells: REST4 silences the silencing activity of neuron-restrictive silencer factor/REST. Mol Cell Biol 19: 6788 – 6795, 1999.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry