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Tet-O-FUW-Ascl1
(Plasmid #27150)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27150 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    TetO-FUW
  • Backbone size w/o insert (bp) 8393
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    The depositing laboratory used JM109 (Promega). The plasmid is provided in NEB Stable to reduce the chance of recombination.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ascl1
  • Alt name
    Mash1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    696
  • Entrez Gene
    Ascl1 (a.k.a. AI225900, ASH1, Mash1, bHLHa46)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-O-FUW-Ascl1 was a gift from Marius Wernig (Addgene plasmid # 27150)
  • For your References section:

    Direct conversion of fibroblasts to functional neurons by defined factors. Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M. Nature. 2010 Feb 25. 463(7284):1035-41. 10.1038/nature08797 PubMed 20107439