Addgene - Tet-O-FUW-Ascl1 Plasmid Data

Plasmid 27150: Tet-O-FUW-Ascl1

Gene/insert name:
Ascl1
Alt name:
Mash1
Insert size:
696
Species:
M. musculus (mouse)
Entrez Gene:
Ascl1 (AI225900, ASH1, Mash1, bHLHa46)
Vector backbone:
TetO-FUW
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
8393
Cloning site 5':
EcoR1
Site destroyed during cloning:
No
Cloning site 3':
EcoR1
Site destroyed during cloning:
No
5' sequencing primer:
LNCX List of Sequencing Primers
3' sequencing primer:
WPRE-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
JM109 (Promega)
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Marius Wernig
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Direct conversion of fibroblasts to functional neurons by defined factors. Vierbuchen et al (Nature. 2010 Feb 25. 463(7284):1035-41. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27150" in your Materials and Methods section.