Addgene - Tet-O-FUW-Olig2 Plasmid Data

Plasmid 30131: Tet-O-FUW-Olig2

Gene/insert name:
Olig2
Insert size:
972
Species:
M. musculus (mouse)
Entrez Gene:
Olig2 (AI604895, Bhlhb1, Olg-2, Oligo2, RK17, bHLHe19)
Vector backbone:
Tet-O-FUW
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
8373
Cloning site 5':
EcoR1
Site destroyed during cloning:
No
Cloning site 3':
EcoR1
Site destroyed during cloning:
No
5' sequencing primer:
LNCX List of Sequencing Primers
3' sequencing primer:
WPRE-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Marius Wernig
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Direct conversion of fibroblasts to functional neurons by defined factors. Vierbuchen et al (Nature. 2010 Feb 25. 463(7284):1035-41. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30131" in your Materials and Methods section.