pCFJ676 - MCS(ttTi4348, I, unc-18(+))
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34872||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeWorm targeting
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namettTi4348 targeting
SpeciesC. elegans (nematode)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer None (Common Sequencing Primers)
Terms and Licenses
Please note that there are TWO M13F primer binding sites. Use custom sequencing primers to verify inserts into MCS.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ676 - MCS(ttTi4348, I, unc-18(+)) was a gift from Erik Jorgensen (Addgene plasmid # 34872 ; http://n2t.net/addgene:34872 ; RRID:Addgene_34872)
For your References section:Improved Mos1-mediated transgenesis in C. elegans. Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM. Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865. 10.1038/nmeth.1865 PubMed 22290181