Addgene - pCFJ676 - MCS(ttTi4348, I, unc-18(+)) Plasmid Data

Plasmid 34872: pCFJ676 - MCS(ttTi4348, I, unc-18(+))

Gene/insert name:
ttTi4348 targeting
Species:
C. elegans (nematode)
Vector backbone:
pDESTR4-R3
(Search Vector Database)
Vector type:
Worm targeting
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
None List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Top10/P3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Erik Jorgensen
Terms and Licenses:
MTA

Comments:

Please note that there are TWO M13F primer binding sites. Use custom sequencing primers to verify inserts into MCS.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Improved Mos1-mediated transgenesis in C. elegans. Frokjaer-Jensen et al (Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34872" in your Materials and Methods section.