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Improved Mos1-mediated transgenesis in C. elegans.
Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
34861pCJF604 - pDESTR4-R3(ttTi4391, I)
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34862pCFJ606 - pDESTR4-R3(ttTi14024, X)
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34863pCFJ210 - pDESTR4-R3(ttTi4348, I)
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34864pCFJ212 - pDESTR4-R3(cxTi10816, IV)
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34865pCFJ448 - pDESTR4-R3(ttTi4348, I, unc-18)
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34866pCFJ350 - MCS(ttTi5605, II)
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34867pCFJ351 - MCS(cxTi10882, IV)
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34868pCFJ352 - MCS(ttTi4348, I)
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34869pCFJ353 - MCS(ttTi4391, I)
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34870pCFJ355 - MCS(ttTi14024, X)
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34871pCFJ356 - MCS(cxTi10816, IV)
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34872pCFJ676 - MCS(ttTi4348, I, unc-18(+))
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34873pMA122 - peel-1 negative selectionNegative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans
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34874pCFJ601 - Peft-3 Mos1 transposaseMos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans
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34875pCFJ326 - operon for germline expression
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34876pCFJ421 - Pmyo-2::GFP::H2B (pharynx)
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34877pCFJ420 - Peft-3::GFP::H2B (ubiquitous)
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34878pCFJ594 - pENTR[1-2](NeoR)
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