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pCJF604 - pDESTR4-R3(ttTi4391, I)
(Plasmid #34861)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34861 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    It is important to grow the bacteria under BOTH Amp and Cm selection.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ttTi4391 targeting
  • Species
    C. elegans (nematode)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

There is a 461 bp deletion in the middle of the left recombination region. This deletion does not appear to adversely affect the ability to insert transgenes into the ttTi4391 site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCJF604 - pDESTR4-R3(ttTi4391, I) was a gift from Erik Jorgensen (Addgene plasmid # 34861 ; http://n2t.net/addgene:34861 ; RRID:Addgene_34861)
  • For your References section:

    Improved Mos1-mediated transgenesis in C. elegans. Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM. Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865. 10.1038/nmeth.1865 PubMed 22290181