pUAS-hActin-luc2
(Plasmid
#48081)
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PurposeLuciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUAS-luc2
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLuciferase
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Alt namepUAS-luc2
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SpeciesH. sapiens (human)
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Mutationremoved hsp70 promoter, inserted amplified 615 bp fragment of the human ACTB promoter
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAS-hActin-luc2 was a gift from Huda Zoghbi (Addgene plasmid # 48081 ; http://n2t.net/addgene:48081 ; RRID:Addgene_48081) -
For your References section:
An AT-hook domain in MeCP2 determines the clinical course of Rett syndrome and related disorders. Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY. Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038. 10.1016/j.cell.2013.01.038 PubMed 23452848