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An AT-hook domain in MeCP2 determines the clinical course of Rett syndrome and related disorders.
Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY
Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
48078pDEST-hMeCP2-GFPMammalian expression of GFP tagged MeCP2-WT
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48079pDEST-hMeCP2-GFP-R270XMammalian expression of GFP tagged MeCP2-R270X
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48080pDEST-hMeCP2-GFP-G273XMammalian expression of GFP tagged MeCP2-G273X
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48081pUAS-hActin-luc2Luciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter
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48082pCMX-Gal4-hMeCP2Mammalian expression of Gal4 tagged MeCP2-WT
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48083pCMX-Gal4-hMeCP2-R270XMammalian expression of Gal4 tagged MeCP2-R270X
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48084pCMX-Gal4-hMeCP2-G273XMammalian expression of Gal4 tagged MeCP2-G273X
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48085pCMX-Gal4-hMeCP2-R111GMammalian expression of Gal4 tagged MeCP2-WT-R111G
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48086pCMX-Gal4-hMeCP2-R270X-R111GMammalian expression of Gal4 tagged MeCP2-R270X-R111G
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48087pCMX-Gal4-hMeCP2-G273X-R111GMammalian expression of Gal4 tagged MeCP2-G273X-R111G
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48089pGEX-5x3-hMeCP2-delN256-R270XBacterial expression of GST tagged AT-Hook2-R270X
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48090pGEX-5x3-hMeCP2-delN256-G273XBacterial expression of GST tagged AT-Hook2-G273X
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48091pTXB1-hMeCP2Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WT
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48092pTXB1-hMeCP2-R270XBacterial expression of Mxe intein/chitin binding domain tagged MeCP2-R270X
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48093pTXB1-hMeCP2-G273XBacterial expression of Mxe intein/chitin binding domain tagged MeCP2-G273X
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