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M-ST1n-VP64
(Plasmid #48675)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48675 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.3 TOPO
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 8998
  • Vector type
    CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9-VP64, nuclease-null
  • Species
    S. thermophilus #1
  • Insert Size (bp)
    3570
  • Mutation
    nuclease-null (D9A D598A H599A N622A)
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • VP64 (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M-ST1n-VP64 was a gift from George Church (Addgene plasmid # 48675)
  • For your References section:

    Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. 10.1038/nmeth.2681 PubMed 24076762