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Purposebroad-host range natural conjugative plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 51950 | Bacteria in agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneRK24
- Total vector size (bp) 60099
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ECNR2.ΔtolC.mutS:zeo [pRK24]
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameE. coli Conjugation genes
- Promoter na
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
LB Lennox with Tetracycline to maintain plasmid, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK24 was a gift from Farren Isaacs (Addgene plasmid # 51950) -
For your References section:
Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Ma NJ, Moonan DW, Isaacs FJ. Nat Protoc. 2014 Oct;9(10):2285-300. doi: 10.1038/nprot.2014.081. Epub 2014 Sep 4. 10.1038/nprot.2014.081 PubMed 25188631
Map uploaded by the depositor.
