Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

rEc2
(Bacterial strain #51946)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 51946 Bacteria in agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    rEc2
  • Copy number
    Unknown

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

LB Lennox, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media. Although its genotype is similar to wild-type ECNR2, it contains regions where TAG has been recoded to TAA.

Genotype: ΔmutS:cam, Δ(ybhB-bioAB):[λcI857.Δ(cro-ea59):tetR-bla]

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rEc2 was a gift from Farren Isaacs (Addgene plasmid # 51946)
  • For your References section:

    Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Ma NJ, Moonan DW, Isaacs FJ. Nat Protoc. 2014 Oct;9(10):2285-300. doi: 10.1038/nprot.2014.081. Epub 2014 Sep 4. 10.1038/nprot.2014.081 PubMed 25188631