Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||51946||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
LB Lennox, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media. Although its genotype is similar to wild-type ECNR2, it contains regions where TAG has been recoded to TAA.
Genotype: ΔmutS:cam, Δ(ybhB-bioAB):[λcI857.Δ(cro-ea59):tetR-bla]
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:rEc2 was a gift from Farren Isaacs (Addgene plasmid # 51946)
For your References section:Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Ma NJ, Moonan DW, Isaacs FJ. Nat Protoc. 2014 Oct;9(10):2285-300. doi: 10.1038/nprot.2014.081. Epub 2014 Sep 4. 10.1038/nprot.2014.081 PubMed 25188631