Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||51948||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)rEc 27.H.Sc
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
LB Lennox, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media. It has 13 "essential" genes that end in TAG recoded to TAA, and its prfA gene has been deleted. Though viable, strain growth is slow.
Genotype: ΔmutS:cat.Δ(ybhB-bioAB):[λcI857.Δ(cro-ea59):tetR-bla], ΔprfA, spec+
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:rEc 27.H.Sc was a gift from Farren Isaacs (Addgene plasmid # 51948)
For your References section:Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Ma NJ, Moonan DW, Isaacs FJ. Nat Protoc. 2014 Oct;9(10):2285-300. doi: 10.1038/nprot.2014.081. Epub 2014 Sep 4. 10.1038/nprot.2014.081 PubMed 25188631