rEc 27.H.Sc
(Bacterial strain
#51948)
-
PurposespecR template
-
Depositing Lab
-
Publication
-
Sequence Information
-
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Bacterial Strain | 51948 | Bacteria in agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backboneNA
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin
-
Growth Temperature30°C
-
Growth Strain(s)rEc 27.H.Sc
-
Copy numberUnknown
Resource Information
-
Terms and Licenses
-
Article Citing this Plasmid
Depositor Comments
LB Lennox, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media. It has 13 "essential" genes that end in TAG recoded to TAA, and its prfA gene has been deleted. Though viable, strain growth is slow.
Genotype: ΔmutS:cat.Δ(ybhB-bioAB):[λcI857.Δ(cro-ea59):tetR-bla], ΔprfA, spec+
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
rEc 27.H.Sc was a gift from Farren Isaacs (Addgene plasmid # 51948)