rEc 27.H.Sc
(Bacterial strain #51948)

No maps are available for this item.

Purpose

specR template
Depositing Lab

Farren Isaacs
Publication

Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi: 10.1038/nprot.2014.081. Epub 2014 Sep 4. ( How to cite )
Sequence Information

Full plasmid sequence is not available for this item.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Bacterial Strain	51948	Bacteria in agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
NA
(Search Vector Database)

Growth in Bacteria

Bacterial Resistance(s)
Spectinomycin
Growth Temperature
30°C
Growth Strain(s)
rEc 27.H.Sc
Copy number
Unknown

Resource Information

Article Citing this Plasmid
- 1 Reference

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

LB Lennox, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media. It has 13 "essential" genes that end in TAG recoded to TAA, and its prfA gene has been deleted. Though viable, strain growth is slow.

Genotype: ΔmutS:cat.Δ(ybhB-bioAB):[λcI857.Δ(cro-ea59):tetR-bla], ΔprfA, spec+

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

rEc 27.H.Sc was a gift from Farren Isaacs (Addgene plasmid # 51948)
For your References section:

Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Ma NJ, Moonan DW, Isaacs FJ. Nat Protoc. 2014 Oct;9(10):2285-300. doi: 10.1038/nprot.2014.081. Epub 2014 Sep 4. 10.1038/nprot.2014.081 PubMed 25188631

rEc 27.H.Sc (Bacterial strain #51948)