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Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering.
Ma NJ, Moonan DW, Isaacs FJ
Nat Protoc. 2014 Oct;9(10):2285-300. doi: 10.1038/nprot.2014.081. Epub 2014 Sep 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51946rEc2oriT-kan template
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51948rEc 27.H.ScspecR template
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51950pRK24broad-host range natural conjugative plasmid
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