Purposebroad-host range natural conjugative plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||51950||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 60099
Growth in Bacteria
Growth Strain(s)ECNR2.ΔtolC.mutS:zeo [pRK24]
Copy numberLow Copy
Gene/Insert nameE. coli Conjugation genes
- Promoter na
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
LB Lennox with Tetracycline to maintain plasmid, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK24 was a gift from Farren Isaacs (Addgene plasmid # 51950)
For your References section:Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Ma NJ, Moonan DW, Isaacs FJ. Nat Protoc. 2014 Oct;9(10):2285-300. doi: 10.1038/nprot.2014.081. Epub 2014 Sep 4. 10.1038/nprot.2014.081 PubMed 25188631