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Purposehelper plasmid for bacterial conjugal DNA transfer
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58495 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRL542
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)HB101
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameencodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
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Alt nameMobColK, M.AvaI, M.Eco47II
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MobColK, M.AvaI, M.Eco47II
Please see attached map for more information on how this vector was constructed and cloning details.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRL528 was a gift from Peter Wolk (Addgene plasmid # 58495 ; http://n2t.net/addgene:58495 ; RRID:Addgene_58495) -
For your References section:
Conjugal transfer of DNA to cyanobacteria. Elhai J, Wolk CP. Methods Enzymol. 1988;167:747-54. 0076-6879(88)67086-8 [pii] PubMed 3148842
Map uploaded by the depositor.
