PurposeExpression vector of rat Tyr (albino) guide RNA
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60966||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2147
Growth in Bacteria
Gene/Insert nameTyrosinase gRNA
SpeciesR. norvegicus (rat)
Insert Size (bp)20
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (not destroyed)
- 3′ cloning site BsaI (not destroyed)
- 5′ sequencing primer M13 forward (Common Sequencing Primers)
Terms and Licenses
Targets a mutation 896G>A in exon 2 of the Tyr gene resulting in an Arg299His missense mutation
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pT7-gRNA:Tyr (albino) was a gift from Tomoji Mashimo (Addgene plasmid # 60966 ; http://n2t.net/addgene:60966 ; RRID:Addgene_60966)
For your References section:Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Yoshimi K, Kaneko T, Voigt B, Mashimo T. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240. 10.1038/ncomms5240 PubMed 24967838