Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Golden Gate TALEN Accessory Pack
(Kit # 1000000030 )

Depositing Lab:   Takashi Yamamoto

This supplemental package contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.

This kit consists of 9 individually packaged plasmids, which are shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs.

Please note that the Golden Gate TALEN and TAL Effector Kit is required to use the plasmids in this accessory pack.

Add to Cart
$ 350 USD + shipping

Available to academics and nonprofits only.

Original Publication

Efficient TALEN construction and evaluation methods for human cell and animal applications. Sakuma T, Hosoi S, Woltjen K, Suzuki K, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S, Yamamoto T. Genes Cells. 2013 Apr;18(4):315-26. doi: 10.1111/gtc.12037. Epub 2013 Feb 6. PubMed PMID 23388034.

Description

This supplemental package contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit. These modified pFUS vectors can reduce the number of module plasmids and improve the success rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing unique shorter N- and C-terminal domains (N153AA, C47AA).

Protocols

Protocol for TALEN construction and evaluation as described in the article.

Yamamoto protocol 306.0 KB

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The plasmid kit used for building TALENs and TALE-TFs was a gift from Takashi Yamamoto (Addgene kit #1000000030)"

Efficient TALEN construction and evaluation methods for human cell and animal applications. Sakuma T, Hosoi S, Woltjen K, Suzuki K, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S, Yamamoto T. Genes Cells. 2013 Apr;18(4):315-26. doi: 10.1111/gtc.12037. Epub 2013 Feb 6. PubMed PMID 23388034.

Kit Contents

These items will be shipped at room temperature:

ID Name
43848 pFUS_A1A
43849 pFUS_A2A
43850 pFUS_A2B
43851 pFUS_A3A
43852 pFUS_A3B
43853 pFUS_A4A
43854 pFUS_A4B
43855 pcDNA-TAL-NC2
43856 pCAGGS-TAL-NC2
Content blocked, you may need to disable your ad-blocker.